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KMID : 0363320060270030725
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Journal of Korean Oriental Internal Medicine
2006 Volume.27 No. 3 p.725 ~ p.736
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Study of The Apoptotic Mechanisms of Gunbibosinhangam-tang on Human Neuroblastoma Cell Line BE2
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Cho Young-Kee
Moon Mi-Hyun
Lee Seong-Kyun
Jeong Hyun-Ae
Lee Jung-Sub
Nam Sang-Kyu
Moon Goo
Shin Sun-Ho
Kim Dong-Woung
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Abstract
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Objective: In order to investigate cell death mechanisms by Gunbibosinhangam-Tang(G.B.H) in cancer cells, the activities of apoptosis signaling pathway were tested in human neuroblastoma cell line BE2.
Methods: Viability of BE2 cells was markedly decreased by treatment of the water extract of G.B.H in a dose-dependent manner. G.B.H-induced cell death was confirmed as apoptosis characterized by chromatin condensation, We tested whether the water extract of G.B.H affects the anti-apoptotic proteins such as Bcl-
Results: Bcl- was uneffected by the addition of the water extract of G.B.H in a time-dependent manner. Cleavage of PARP(poly-ADP-ribose polymerase) by activation of caspase-8 protease was also observed in BE2 cells by the treatment of the water extract of G.B.H.
Conclusion: These results suggest that the water extract of G.B.H exerts anti-cancer effects on human neuroblastoma BE2 cells by inducing the apoptotic death via activation of intrinsic caspase cascades.
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KEYWORD
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apoptosis, neuroblastoma, Gunbibosinhangam-Tang
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